Forensic DNA: Miniaturization and Automation

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Description

New technologies are being developed to miniaturize DNA testing instruments and to improve automation of the processes involved. Both miniaturization and automation have the potential to improve the speed and to reduce the cost of DNA analysis. In addition, miniaturizing the instrumentation required to perform DNA testing could enable examination of biological samples at the crime scene if desired in the future here in the United States.

Research Projects

The table below presents awards funded both prior to and under the DNA Initiative, which began in 2004.

Title Awardee Amount Award Number
Use of Novel Chemistry & Microwave Instrumentation to ImproveBody Fluid Assay Sensitivity & Speed while Reducing Costs City of New York, Office of Chief Medical Examiner 2012-DN-BX-K044 $403,671
DNA Analysis of LCN Samples: Towards Fully Integrated STR Profiling Network Biosystems, Inc 2009-DN-BX-K256 $496,991
A Low Cost Microfluidic Instrument for Typing SNPs Akonni Biosystems Inc 2009-DN-BX-K189 $495,031
Microchip Analyzer for Forensic Short Tandem Repeat Typing of Single Cells Regents of the University of California - Berkeley 2009-DN-BX-K180 $241,117
Closed System DNA Purification for Degraded and Compromised Evidence in Microfluidic Devices University of Virginia 2008-DN-BX-K144 $480,000
Sample Collection Systems for DNA Analysis of Forensic Evidence Samples Network Biosystems, Inc. 2008-DN-BX-K010 $498,563
Human Specific DNA Quantitation for Fully Integrated Microfluidic STR Analysis Network Biosystems Inc. 2008-DN-BX-K009 $494,319
Microfluidic DNA Extraction and Purification from Forensic Samples: Towards Rapid, Full Integrated STR Analysis Network Biosystems, Inc. 2007-DN-BX-K184 $497,346
A Low-Cost Microfluidic Microarray Instrument for Typing Y-Chromosome Single Nucleotide Polymorphisms (SNPs) Akonni Biosystems, Inc. 2007-DN-BX-K145 $448,466
Microfabricated Capillary Array Electrophoresis Genetic Analysis for Forensic Short Tandem Repeat DNA Profiling Regents of the University of California 2007-DN-BX-K142 $592,183
Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High-Efficiency, High-Purity Separations University of Virginia 2006-DN-BX-K021 $324,104
SNP-Based Microarray Technology for Use in Forensic Applications Affymetrix, Inc. 2006-DN-BX-K021 $638,098
Analysis of DNA Forensic Markers Using High Throughput Mass Spectrometry Isis Pharmaceuticals, Inc. 2006-DN-BX-K011 $451,382
SNP-Based Microarray Technology for Use in Forensic Applications  Affymetrix, Inc.  2005-DA-BX-K101  $638,098
Electro-Elution Assisted Purification of DNA for Forensic Analysis University of Virginia 2005-DA-BX-K100 $20,000
Nanotechnology DNA Sequencing: Improving DNA Processing Technologies Brown University 2004-LT-BX-K001 $445,265
Nanotechnology DNA Sequencing: Improving DNA Processing Technologies Brown University 2004-LT-BX-K001 $542,654
Microfabricated Capillary Array Electrophoresis Genetic Analyzers for Forensic Short Tandem Repeat DNA Profiling University of California 2004-DN-BX-K216 $821,623
Laser Microdissection Separation of Pure Spermatozoa Populations From Mixed Cell Samples for Forensic DNA Analysis Rosalind Franklin University of Medicine and Science 2004-DN-BX-K215 $449,829
A Hand-Held DNA-Based Forensic Tool Cornell University 2004-DN-BX-K001 $644,251
Application of a Single Robotics Platform to the Combined Extraction, Purification, Quantification, and Amplification of Forensic DNA Samples North Louisiana Criminalistics Laboratory 2002-IJ-CX-K019 $42,401
Program Acceleration and Enhancements for Microdevice DNA Forensics System Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $149,964
Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $250,000
Program Acceleration/Enhancements for Microdevice DNA Forensics System Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $499,342
Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $899,110
Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $1,102,790
Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics Whitehead Institute for Biomedical Research 1998-LB-VX-K022 $2,486,876
Chip-Based Genetic Detector for Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $224,110
Chip Based Genetic Detector for Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $499,882
Chip-Based Genetic Detector for Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $514,848
Chip Based Genetic Detector for Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $709,919
Chip-Based Genetic Detector for Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $799,692
Chip-Based Genetic Detector of Rapid Identification of Individuals Nanogen, Inc. 1997-LB-VX-0004 $1,700,000
Improved Analysis of DNA Short Tandem Repeats for Human Identification Genetrace Systems, Inc. 1997-LB-VX-0003 $301,999
Improved Analysis of DNA Short Tandem Repeats for Human Identification Genetrace Systems, Inc. 1997-LB-VX-0003 $307,664

Research Articles and Reports

Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.

Title Authors Publication Year Awardee Award Number
A Low Cost Microfluidic Microarray System for Typing Y Chromosome SNPs (pdf, 45 pages) Jennifer E. Reynolds, Ph.D., Phil Belgrader Ph.D., Rebecca Holmberg Ph.D., Cynthia, Zimmerman, Clare Stewart, Rebecca Mokhiber, and Christopher Cooney, Ph.D. Final Grant Report 2009 Akonni Biosystems, Inc. 2007-DN-BX-K145
A Hand-Held DNA-Based Forensic Tool (pdf, 55 pages) Carl A. Batt, Scott J. Stelick, Matthew J. Kennedy, Clarissa S. Lui, Adam J. Lowe Final Grant Report 2009 Cornell University 2004-DN-BX-K001
Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High Efficiency, High Purity Separations (pdf, 71 pages) James P. Landers, Ph.D. Final Grant Report 2009 University of Virginia 2006-DN-BX-K021
Integrated sample cleanup-capillary electrophoresis microchip for high-performance short tandem repeat genetic analysis. Yeung SH, Liu P, Del Bueno N, Greenspoon SA, Mathies RA. Anal Chem. 2009 Jan 1;81(1):210-7. 2009 University of California, Berkeley 2007-DN-BX-K142
Microfluidic DNA Extraction and Purification from Forensic Samples (pdf, 88 pages) Eugene Tan, Heidi Giese, and Daniel M. Hartmann Final Grant Report 2009 Network Biosystems, Inc. 2007-DN-BX-K184
A forensic laboratory tests the Berkeley microfabricated capillary array electrophoresis device Greenspoon SA, Yeung SH, Johnson KR, Chu WK, Rhee HN, McGuckian AB, Crouse CA, Chiesl TN, Barron AE, Scherer JR, Ban JD, Mathies RA. J Forensic Sci. 2008 Jul;53(4):828-37 2008 University of California, Berkeley 2004-DN-BX-K216
Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer Peng Liu, Stephanie H.I. Yeung, Karin A. Crenshaw, Cecelia A. Crouse, James R. Scherer, Richard A. Mathies Forensic Science International: Genetics
September 2008 (Vol. 2, Issue 4, Pages 301-309)
2008 University of California, Berkeley 2004-DN-BX-K216
Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing. Liu P, Seo TS, Beyor N, Shin KJ, Scherer JR, Mathies RA. Anal Chem. 2007 Mar 1; 79(5):1881-9. Epub 2007 Feb 2. 2007 University of California, Berkeley 2004-DN-BX-K216
Development of Microdevice Solid-Phase Purification Utilizing Dual Pressure/ Electro-Elution for Concentration and Enhanced Recovery of DNA (pdf, 24 pages) Joan Bienvenue Final Grant Report 2007 University of Virginia 2005-DA-BX-K100
Forensic application of the Affymetrix human mitochondrial resequencing array Vallone, P.M., Jakupciak, J.P., Coble, M.D. Forensic Science International: Genetics
June 2007 (Vol. 1, Issue 2, Pages 196-198)
2007 NIST 2003-IJ-R-029
A Chip-Based Genetic Detector for Rapid Identification of Individuals (pdf, 102 pages) Ron Sosnowski Final Grant Report 2006 Nanogen, Inc. 1997-LB-VX-0004
Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array electrophoresis microdevice. Yeung SH, Greenspoon SA, McGuckian A, Crouse CA, Emrich CA, Ban J, Mathies RA. J Forensic Sci. 2006 Jul; 51(4):740-7. 2006 University of California 2004-DN-BX-K216
Microdevice DNA forensics by the simple tandem repeat method. Goedecke N, McKenna B, El-Difrawy S, Gismondi E, Swenson A, Carey L, Matsudaira P, Ehrlich DJ. J Chromatogr A. 2006 Apr 14; 1111(2):206-13. 2006 Whitehead Institute for Biomedical Research 1998-LB-VX-K022
Surface modification of poly(methyl methacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis. Shah JJ, Geist J, Locascio LE, Gaitan M, Rao MV, Vreeland WN. Electrophoresis. 2006 Oct; 27(19):3788-96. 2006 NIST 2003-IJ-R-029
Cellular immobilization within microfluidic microenvironments: dielectrophoresis with polyelectrolyte multilayers. Forry SP, Reyes DR, Gaitan M, Locascio LE. J Am Chem Soc. 2006 Oct 25; 128(42):13678-9. 2006 NIST 2003-IJ-R-029
Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis. Butler JM, Buel E, Crivellente F, McCord BR. Electrophoresis. 2004 Jun; 25(10-11):1397-412. 2004 National Institute of Standards and Technology 1999-IJ-R-A094
Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS. Vallone PM, Fahr K, Kostrzewa M. Methods Mol Biol. 2004; 297:169-78. 2004 National Institute of Standards and Technology 1999-IJ-R-A094
Multiple sample amplification and genotyping integrated on a single electronic microarray. Huang Y, Shirajian J, Schroder A, Yao Z, Summers T, Hodko D, Sosnowski R. Electrophoresis. 2004 Oct: 25(18-19): 3106-16 2004 Nanogen, Inc. 1997-LB-VX-0004
A high-performance multilane microdevice system designed for the DNA forensics laboratory. Goedecke N, McKenna B, El-Difrawy S, Carey L, Matsudaira P, Ehrlich D. Electrophoresis. 2004 Jun; 25(10-11):1678-86. 2004 Whitehead Institute for Biomedical Research 1998-LB-VX-K022
High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice. Mitnik L, Carey L, Burger R, Desmarais S, Koutny L, Wernet O, Matsudaira P, Ehrlich D. Electrophoresis. 2002 Mar; 23(5):719-26. 2002 Whitehead Institute for Biomedical Research 1998-LB-VX-K022
Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip. Radtkey R, Feng L, Muralhidar M, Duhon M, Canter D, DiPierro D, Fallon S, Tu E, McElfresh K, Nerenberg M, Sosnowski R. Nucleic Acids Res. 2000 Apr 1; 28(7):E17. 2000 Nanogen, Inc. 1997-LB-VX-0004
Reliable genotyping of short tandem repeat loci without an allelic ladder using time-of-flight mass spectrometry. Butler JM, Li J, Shaler TA, Monforte JA, Becker CH. Int J Legal Med. 1999; 112(1):45-9 1999 Genetrace Systems, Inc. 1997-LB-VX-0003
Two-color multiplexed analysis of eight short tandem repeat loci with an electrophoretic microdevice. Schmalzing D, Koutny L, Chisholm D, Adourian A, Matsudaira P, Ehrlich D. Anal Biochem. 1999 May 15; 270(1):148-52. 1999 Whitehead Institute for Biomedical Research 1998-LB-VX-K022
Single nucleotide polymorphism determination using primer extension and time-of-flight mass spectrometry. Li J, Butler JM, Tan Y, Lin H, Royer S, Ohler L, Shaler TA, Hunter JM, Pollart DJ, Monforte JA, Becker CH. Electrophoresis. 1999 Jun; 20(6):1258-65. 1999 Genetrace Systems, Inc. 1997-LB-VX-0003
Date Modified: October 11, 2012